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anti fcov nucleocapsid  (Bio-Rad)


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    Structured Review

    Bio-Rad anti fcov nucleocapsid
    Anti Fcov Nucleocapsid, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fcov+nucleocapsid/Mouse+anti+Feline+Coronavirus/pmc13088912-83-68-76
    Average 93 stars, based on 41 article reviews
    anti fcov nucleocapsid - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Formalin-fixed Paraffin-Embedded:

    Article Title: Beyond macrophages: FIPV tropism includes T and B lymphocytes.
    Article Snippet: .. FFPE lymph node tissue sections were deparaffinized prior to antigen retrieval using boiling commercially available buffer (DAKO Target Antigen Retrieval Solution, pH 6.1, cat# S2375), followed by blocking using donkey serum at room temperature for 1 h. Sections were then incubated with primary antibodies, including anti-CD20 (Polyclonal Rabbit anti-human; 1:100; Invitrogen, cat#16701), anti-CD3 (CA17.2A12 rat anti-canine; 1:10; Leukocyte Antigen Biology Laboratory), anti-IBA1 (Polyclonal Rabbit anti-IBA1;1:100; FUJIFILM, cat#019–19741) and anti-FCoV nucleocapsid (monoclonal Mouse antiFeline corona virus; 1:100; BIORAD, cat# MCA2194). .. After overnight incubation at 4◦C, the slides were washed with Tris buffered saline with tween 20 (TBST) and stained with secondary antibodies: Alexa Fluor 488 donkey anti-rabbit (Invitrogen, cat# A21206), Alexa Fluor 647 donkey anti-rat (Invitrogen, cat# A48272), and Alexa Fluor 594 donkey anti-mouse (Invitrogen, cat# A21203) at room temperature for 1 h. Cell nuclei were visualized using DAPI nuclear counterstain (Invitrogen, cat# D1306), and slides were mounted using ProLong Gold Antifade Mountant (Thermo Fisher Scientific, cat# P36934).

    Article Title: Beyond macrophages: FIPV tropism includes T and B lymphocytes
    Article Snippet: .. FFPE lymph node tissue sections were deparaffinized prior to antigen retrieval using boiling commercially available buffer (DAKO Target Antigen Retrieval Solution, pH 6.1, cat# S2375), followed by blocking using donkey serum at room temperature for 1 h. Sections were then incubated with primary antibodies, including anti-CD20 (Polyclonal Rabbit anti-human; 1:100; Invitrogen, cat#16701), anti-CD3 (CA17.2A12 rat anti-canine; 1:10; Leukocyte Antigen Biology Laboratory), anti-IBA1 (Polyclonal Rabbit anti-IBA1;1:100; FUJIFILM, cat#019-19741) and anti-FCoV nucleocapsid (monoclonal Mouse anti-Feline corona virus; 1:100; BIORAD, cat# MCA2194). .. After overnight incubation at 4°C, the slides were washed with Tris buffered saline with tween 20 (TBST) and stained with secondary antibodies: Alexa Fluor 488 donkey anti-rabbit (Invitrogen, cat# A21206), Alexa Fluor 647 donkey anti-rat (Invitrogen, cat# A48272), and Alexa Fluor 594 donkey anti-mouse (Invitrogen, cat# A21203) at room temperature for 1 h. Cell nuclei were visualized using DAPI nuclear counterstain (Invitrogen, cat# D1306), and slides were mounted using ProLong Gold Antifade Mountant (Thermo Fisher Scientific, cat# P36934).

    Blocking Assay:

    Article Title: Beyond macrophages: FIPV tropism includes T and B lymphocytes.
    Article Snippet: .. FFPE lymph node tissue sections were deparaffinized prior to antigen retrieval using boiling commercially available buffer (DAKO Target Antigen Retrieval Solution, pH 6.1, cat# S2375), followed by blocking using donkey serum at room temperature for 1 h. Sections were then incubated with primary antibodies, including anti-CD20 (Polyclonal Rabbit anti-human; 1:100; Invitrogen, cat#16701), anti-CD3 (CA17.2A12 rat anti-canine; 1:10; Leukocyte Antigen Biology Laboratory), anti-IBA1 (Polyclonal Rabbit anti-IBA1;1:100; FUJIFILM, cat#019–19741) and anti-FCoV nucleocapsid (monoclonal Mouse antiFeline corona virus; 1:100; BIORAD, cat# MCA2194). .. After overnight incubation at 4◦C, the slides were washed with Tris buffered saline with tween 20 (TBST) and stained with secondary antibodies: Alexa Fluor 488 donkey anti-rabbit (Invitrogen, cat# A21206), Alexa Fluor 647 donkey anti-rat (Invitrogen, cat# A48272), and Alexa Fluor 594 donkey anti-mouse (Invitrogen, cat# A21203) at room temperature for 1 h. Cell nuclei were visualized using DAPI nuclear counterstain (Invitrogen, cat# D1306), and slides were mounted using ProLong Gold Antifade Mountant (Thermo Fisher Scientific, cat# P36934).

    Article Title: Beyond macrophages: FIPV tropism includes T and B lymphocytes
    Article Snippet: .. FFPE lymph node tissue sections were deparaffinized prior to antigen retrieval using boiling commercially available buffer (DAKO Target Antigen Retrieval Solution, pH 6.1, cat# S2375), followed by blocking using donkey serum at room temperature for 1 h. Sections were then incubated with primary antibodies, including anti-CD20 (Polyclonal Rabbit anti-human; 1:100; Invitrogen, cat#16701), anti-CD3 (CA17.2A12 rat anti-canine; 1:10; Leukocyte Antigen Biology Laboratory), anti-IBA1 (Polyclonal Rabbit anti-IBA1;1:100; FUJIFILM, cat#019-19741) and anti-FCoV nucleocapsid (monoclonal Mouse anti-Feline corona virus; 1:100; BIORAD, cat# MCA2194). .. After overnight incubation at 4°C, the slides were washed with Tris buffered saline with tween 20 (TBST) and stained with secondary antibodies: Alexa Fluor 488 donkey anti-rabbit (Invitrogen, cat# A21206), Alexa Fluor 647 donkey anti-rat (Invitrogen, cat# A48272), and Alexa Fluor 594 donkey anti-mouse (Invitrogen, cat# A21203) at room temperature for 1 h. Cell nuclei were visualized using DAPI nuclear counterstain (Invitrogen, cat# D1306), and slides were mounted using ProLong Gold Antifade Mountant (Thermo Fisher Scientific, cat# P36934).

    Incubation:

    Article Title: Beyond macrophages: FIPV tropism includes T and B lymphocytes.
    Article Snippet: .. FFPE lymph node tissue sections were deparaffinized prior to antigen retrieval using boiling commercially available buffer (DAKO Target Antigen Retrieval Solution, pH 6.1, cat# S2375), followed by blocking using donkey serum at room temperature for 1 h. Sections were then incubated with primary antibodies, including anti-CD20 (Polyclonal Rabbit anti-human; 1:100; Invitrogen, cat#16701), anti-CD3 (CA17.2A12 rat anti-canine; 1:10; Leukocyte Antigen Biology Laboratory), anti-IBA1 (Polyclonal Rabbit anti-IBA1;1:100; FUJIFILM, cat#019–19741) and anti-FCoV nucleocapsid (monoclonal Mouse antiFeline corona virus; 1:100; BIORAD, cat# MCA2194). .. After overnight incubation at 4◦C, the slides were washed with Tris buffered saline with tween 20 (TBST) and stained with secondary antibodies: Alexa Fluor 488 donkey anti-rabbit (Invitrogen, cat# A21206), Alexa Fluor 647 donkey anti-rat (Invitrogen, cat# A48272), and Alexa Fluor 594 donkey anti-mouse (Invitrogen, cat# A21203) at room temperature for 1 h. Cell nuclei were visualized using DAPI nuclear counterstain (Invitrogen, cat# D1306), and slides were mounted using ProLong Gold Antifade Mountant (Thermo Fisher Scientific, cat# P36934).

    Article Title: Beyond macrophages: FIPV tropism includes T and B lymphocytes
    Article Snippet: .. FFPE lymph node tissue sections were deparaffinized prior to antigen retrieval using boiling commercially available buffer (DAKO Target Antigen Retrieval Solution, pH 6.1, cat# S2375), followed by blocking using donkey serum at room temperature for 1 h. Sections were then incubated with primary antibodies, including anti-CD20 (Polyclonal Rabbit anti-human; 1:100; Invitrogen, cat#16701), anti-CD3 (CA17.2A12 rat anti-canine; 1:10; Leukocyte Antigen Biology Laboratory), anti-IBA1 (Polyclonal Rabbit anti-IBA1;1:100; FUJIFILM, cat#019-19741) and anti-FCoV nucleocapsid (monoclonal Mouse anti-Feline corona virus; 1:100; BIORAD, cat# MCA2194). .. After overnight incubation at 4°C, the slides were washed with Tris buffered saline with tween 20 (TBST) and stained with secondary antibodies: Alexa Fluor 488 donkey anti-rabbit (Invitrogen, cat# A21206), Alexa Fluor 647 donkey anti-rat (Invitrogen, cat# A48272), and Alexa Fluor 594 donkey anti-mouse (Invitrogen, cat# A21203) at room temperature for 1 h. Cell nuclei were visualized using DAPI nuclear counterstain (Invitrogen, cat# D1306), and slides were mounted using ProLong Gold Antifade Mountant (Thermo Fisher Scientific, cat# P36934).

    Virus:

    Article Title: Beyond macrophages: FIPV tropism includes T and B lymphocytes.
    Article Snippet: .. FFPE lymph node tissue sections were deparaffinized prior to antigen retrieval using boiling commercially available buffer (DAKO Target Antigen Retrieval Solution, pH 6.1, cat# S2375), followed by blocking using donkey serum at room temperature for 1 h. Sections were then incubated with primary antibodies, including anti-CD20 (Polyclonal Rabbit anti-human; 1:100; Invitrogen, cat#16701), anti-CD3 (CA17.2A12 rat anti-canine; 1:10; Leukocyte Antigen Biology Laboratory), anti-IBA1 (Polyclonal Rabbit anti-IBA1;1:100; FUJIFILM, cat#019–19741) and anti-FCoV nucleocapsid (monoclonal Mouse antiFeline corona virus; 1:100; BIORAD, cat# MCA2194). .. After overnight incubation at 4◦C, the slides were washed with Tris buffered saline with tween 20 (TBST) and stained with secondary antibodies: Alexa Fluor 488 donkey anti-rabbit (Invitrogen, cat# A21206), Alexa Fluor 647 donkey anti-rat (Invitrogen, cat# A48272), and Alexa Fluor 594 donkey anti-mouse (Invitrogen, cat# A21203) at room temperature for 1 h. Cell nuclei were visualized using DAPI nuclear counterstain (Invitrogen, cat# D1306), and slides were mounted using ProLong Gold Antifade Mountant (Thermo Fisher Scientific, cat# P36934).

    Article Title: Beyond macrophages: FIPV tropism includes T and B lymphocytes
    Article Snippet: .. FFPE lymph node tissue sections were deparaffinized prior to antigen retrieval using boiling commercially available buffer (DAKO Target Antigen Retrieval Solution, pH 6.1, cat# S2375), followed by blocking using donkey serum at room temperature for 1 h. Sections were then incubated with primary antibodies, including anti-CD20 (Polyclonal Rabbit anti-human; 1:100; Invitrogen, cat#16701), anti-CD3 (CA17.2A12 rat anti-canine; 1:10; Leukocyte Antigen Biology Laboratory), anti-IBA1 (Polyclonal Rabbit anti-IBA1;1:100; FUJIFILM, cat#019-19741) and anti-FCoV nucleocapsid (monoclonal Mouse anti-Feline corona virus; 1:100; BIORAD, cat# MCA2194). .. After overnight incubation at 4°C, the slides were washed with Tris buffered saline with tween 20 (TBST) and stained with secondary antibodies: Alexa Fluor 488 donkey anti-rabbit (Invitrogen, cat# A21206), Alexa Fluor 647 donkey anti-rat (Invitrogen, cat# A48272), and Alexa Fluor 594 donkey anti-mouse (Invitrogen, cat# A21203) at room temperature for 1 h. Cell nuclei were visualized using DAPI nuclear counterstain (Invitrogen, cat# D1306), and slides were mounted using ProLong Gold Antifade Mountant (Thermo Fisher Scientific, cat# P36934).



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